Computational Structural Analysis and Homology Modelling of Beta-Xylanase from Bifidobacterium pullorum: A Comprehensive In-Silico Investigation

Abdul Qadeer Baseer; Shafiqullah Mushfiq; Abdul Wahid Monib; Mohammad Hassan Hassand; Parwiz Niazi

doi:10.55544/jrasb.2.6.9

Authors

Abdul Qadeer Baseer Department of Biology, Faculty of Education, Kandahar University, Kandahar, AFGHANISTAN.
Shafiqullah Mushfiq Department of Biology, Faculty of Education, Kandahar University, Kandahar, AFGHANISTAN.
Abdul Wahid Monib Department of Biology, Faculty of Education, Kandahar University, Kandahar, AFGHANISTAN.
Mohammad Hassan Hassand Faculty of Biology and biotechnology, Al Farabi Kazakh National University, 71 Al-Farabi Ave, Almaty 050040, KAZAKHSTAN.
Parwiz Niazi Department of Biology, Faculty of Education, Kandahar University, Kandahar, AFGHANISTAN.

DOI:

https://doi.org/10.55544/jrasb.2.6.9

Keywords:

Mesophilic, Beta-xylanase, Biogas, Xylan, Bio-renewable energy, Genomic

Abstract

Bifidobacterium pullorum, commonly found in chicken waste and preferring mesophilic characteristics, contains an enzyme known as Beta-Xylanase. This enzyme effectively breaks down xylan, offering potential for creating biogas, like methane, and biofuels, such as ethanol. Scientists are actively exploring sustainable energy sources, while industries aim for cost-effective methods to decrease operational expenses. The conventional methods for producing biogas and biofuels involve high-temperature processes using fuel combustion, leading to significant expenses. To address this, mesophilic bacteria present a promising alternative for more cost-efficient biofuel production. This study is the first to delve into the genomic and three-dimensional structure of beta-xylanase, crucial for breaking down xylan. Our findings highlight that the beta-xylanase in Bifidobacterium pullorum showcases a TIM-barrel structure, similar to other GH10 xylanases essential in carbohydrate breakdown. This indicates a potential connection between Bifidobacterium pullorum's beta-xylanase and the improvement of biogas production.

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