Regeneration of Cowpea Vigna radiata L. Plant from Cell Suspension

Authors

  • Ghanyah Hiatham Al-Qasab Department of Biology, College of Education for Pure Sciences, University of Mosul, IRAQ.
  • Jamella Hazza Rasheed Department of Biology, College of Education for Pure Sciences, University of Mosul, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.2.1.25

Keywords:

Cowpea (Vigna radiata L.), Cell suspension cultures, NAA (Naphthalen acetic acid), BA (Benzyl adenine), MS (Murashige & Skoog medium)

Abstract

This study dealt with the establishment of cell suspensions culture from cotyledonous stems callus of Vigna radiata L., using an MS medium supplemented with 1.0 mg-1 Naphthalene acetic acid (NAA) and 0.5 mg-1 Benzyl adenine (BA). Two densities of cell suspensions were grown using two types of media. The first medium, MS0, was devoid of growth regulators. The density of the third day was higher than that of the fourth day. The other medium is MS containing growth regulators, and the third-day density was also higher than the fourth-day density in culture suspensions. the cell density grown in MS medium supplemented with growth regulators is higher than the density of cells growing in MS0 medium devoid of growth regulators.

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References

Al-Badrani, Sabah Abdullah Salih and Al-Bakr, Rehab Abdel-Jabbar Hamed, (2021). Creation of callus from plant cuttings of the seedlings of the camel thistle plant Silybum marianum and estimation of their protein content, Al-Rafidain Sciences Journal, 30: 21_37.

Al-Bayati, F. A. Y. (2002). Genetic changes in seeds, plants, and callus of soybean Glycine max (parent variety) using gamma rays and their effects on protein content and oil content. M.Sc. Thesis, University of Mosul.

Al-Jwari, S. M. (2004). Co– the cultivation of cell suspension derived from stems callus with Ri plasmids in obtaining transgenic Vicia faba L. broad bean plants. Ph.D. thesis, University of Mosul.

Arife, K. and Muhammed, A. (2019). Thidiazuron (TDZ) Induced In vitro axillary shoot regeneration of Desi chickpea (Cicer arietinum L.). Journal of Applied Biological Sciences., 13 (1): 17-20.

Aparna, P. P.; Kailash Ch. S.; Gyana R. R.; Simachal S. and Prem N. J., (2018). In vitro Regeneration of recalcitrant green gram (Vigna radiata L. Wilczek) from Immature cotyledons for Genetic Improvement. Int. J. Curr. Microbiol. App. Sci 7(1): 3072-3080.

Bharathi P. and Elavarasi N. (2012). Preliminary studies of reactor system designed for cell suspension culture of chickpea (Cicer arietinum L.) International J. of Chem. Sci. and App.3(1):223-231.

Binding, H. and Nehls, R. (1978). Regeneration of isolated protoplasts of Vicia faba L. Z. Pflanzenphysiol. Bd., 88. S. 327- 332

Birkenhead, K. and Willmer, C.M. (1986). Some biochemical characteristics of guard cell and mesophyll cell protoplasts from Commelina communis L. J. Exp.Bot.37:119-128.

Dietz, A; Kutschera, U. and Ray, P.M. (1990). Auxin enhancement of mRNAs in the epidermis and internal tissue in the pea stem and its significance for the control of elongation. Plant Physiol.,23:432- 438.

Gresshoff PM. (1980). In vitro culture of white clover: callus, suspension, protoplast culture, and plant regeneration. Bot. Gazetta, 141(2):157-164.

Havenith, H.; Raven, N.; Di Fire, S. Fischer, R. and Schillberge, S. (2014). Image–based analysis of cell–specific productivity for plant cell suspension cultures. Plant cell, Tissue, and Organ Culture. 117: 393-399.

Hernandez – Garcia C. velazouez – Becerra R. Herrera – Bucio JG. acia – Magana. P. Lopez and Albarran E. (2021). Establishment of callus and cell suspensions cultures of Dalbergiia congest flora (Fabaceae) to Mediacarpin production. Asian J. Plant Sci., 20 (1): 109-115.

Khatun, M. K., Haque, M. S., Islam, S. and Nasiruddin, M. 2008. In vitro regeneration of mung bean (Vigna radiata L.) from different explants. Progress. Agric. 19(2): 13-19.

Mohammed, A.A.; Al-Mallah, M.K. (2013). Determination of β-carotene in Carrot (Daucus carota L.) plants regenerated from stems callus. Raf. J. Sci., 24, 27-36.

Morris, P. and Fowler, M.W. (1981). A new method for the production of fine plant cell suspension culture. Plant Cell, Tissue and Organ Culture.1(1): 15-24.

Moscatiello, R.; Baldan, B. and Navazio, L. (2013). Pant cell suspension culture. Methods Mol.Bio.,953:77 –93.

Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15(3): 473-497.

Neumann, K.; Kumar, A. and Imani, J. (2009). Plant Cell and Tissue Culture – A Tool in Biotechnology. Springer-Verlag Berlin, Germany.

Paul J., 1970. Cell and Tissue Culture. London.

Raghavendra T, et al.,2018. An efficient in vitro shoot regeneration protocol from embryo explants of chickpea (Cicer arietinum L.) Legume Research an International Journal,3844(2):1-4.

Rasheed, J. H., Qassem, and W. S. (2006) The role of heat treatment in stimulating cell division and callus formation from the cultivation of cell suspensions of Helianthus annuus L. in multiple agar drops. Al-Rafidain Agricultural Journal, 8-1:34 (2):8-1.

RB. Santos; R. Abranches ; R. Fischer ; M. Sack and T. Holland T. (2016). Putting the spotlight back on plant suspension cultures. Frontiers in Plant Science. 7: 297.

Satdive, R., Shinde, A. N., Singh, S., Kamble, S., Singh, S., Malpathak, N., and Fulzele, D. P. (2015). Aggregate cell suspension cultures of Psoralea corylifolia improved phytoestrogens production. Biotechnology and Bioprocess Engineering. 20: 373-379.

Sheahan, C.M., (2012). Plant guide for cowpea (Vigna unguiculata). USDA-Natural Resources Conservation Service, Cape May Plant Materials Center, Cape May, NJ.

Silveira, V.; Floh, E.I. S.; Handro, W. and Guerra, M.P. (2009). Effect of plant growth regulators on the cellular growth and levels of intercellular protein, starch and polyamines in embryogenic suspension culture of Pinus taeda L. Plant Cell, Tiss,& Org. Cult.,76:53 –60.

Varalaxmi, Y., Vijaylaxmi, A., Yadav, S. K., Venketeswaralu, B. and Maheswari M. (2007). Efficient plant regeneration from cotyledons of black gram [Vigna mungo (L.) Hepper]. Indian J. Biotechnol., 6: 414-417

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Published

2023-02-25

How to Cite

Al-Qasab, G. H., & Rasheed, J. H. (2023). Regeneration of Cowpea Vigna radiata L. Plant from Cell Suspension. Journal for Research in Applied Sciences and Biotechnology, 2(1), 179–185. https://doi.org/10.55544/jrasb.2.1.25