Effect of Temperature on the Ability of Pseudomonas stutzeri Bacteria Isolated from Different Sources to Fix Nitrogen

Abstract

Background Research has tended to increase the number of nitrogen-fixing microbes in the ground to increase the rate of Stabilization and supply plants with their needs of Nitrogen while reducing nitrogen fertilization and the surrounding problems of pollution, loss, and cost, so a type of microorganism was used in this degree to fix Nitrogen and its use as an alternative to chemical fertilizers.

Methodology, the isolated Pseudomonas stutzeri bacteria from different sources, were planted on a nitrogen-free medium after sterilization in an autoclave device, and the medium was distributed in 10 ml tubes. Then the bacteria were cultured in this medium and incubated for five days at a temperature of 37 °C, after which the growth was measured by a spectrometer and using the Nessler reagent detector. The same nitrogen-free medium was used, but the bacteria were incubated at different degrees. They were divided into three groups, the first incubated at 25 °C, the second at 35 °C, and the last at 45 °C.

The effect of isolating Pseudomonas stutzeri bacteria from different sources on nitrogen fixation was isolated from urine, feces, and sputum. The nitrogen concentration in the medium containing the bacteria isolated from urine was 3.8%. In contrast, the Nitrogen installed in the medium containing the bacteria isolated from the feces reached the highest concentration of 6.2. The nitrogen fixation in the medium containing the bacteria isolated from the sputum reached the lowest concentration of 2.4%, As in Figure 1 This case can be explained by the fact that the presence of bacteria in the intestine led to the gene expression of the genes that encode the enzyme Nitrogenase. Effect of temperature difference on nitrogen fixation in the culture media. The treatments were divided into three treatments, with five replications for each treatment after sterilization of the culture media. They were distributed into 10 ml tubes and inoculum with bacteria for each tube. The first group was incubated at 25 °C, the second at 35 °C, and the last at 45 °C. For five days in a vibrating incubator, the results showed that the concentration of Nitrogen installed in the first treatment (25 °C) was 2%, while the highest concentration in the second treatment (35 °C) was 5.2%, and in the last treatment (45 °C) 3.4%, as shown in Figure 2. Interpretation of this is that the ideal degree for bacterial growth is 37 °C.