Study the Efficiency of Some Antibiotics on Staph. aureus Isolated from different Clinical Origins

Fanar Dawas Mahmood; Ibrahim Omar Saeed; Thamer Abdullah Zahwan

doi:10.55544/jrasb.1.4.18

Authors

Fanar Dawas Mahmood Department of Biology, College of Science, Tikrit University, IRAQ.
Ibrahim Omar Saeed Department of Biology, College of Science, Tikrit University, IRAQ.
Thamer Abdullah Zahwan Department of Horticulture and Landscape, College of Agriculture and Forestry, Tikrit University, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.1.4.18

Keywords:

Staphylococcus aureus, antibiotics, ß-lactamases

Abstract

For the period from April to October 2022, (56) isolates of bacteria infected with Staphylococcus aureus were diagnosed, distributed among 5 isolates of aureus infection, out of a total of (23) isolates (41%) and (33) isolates of burn injuries. Of the total (56) isolates, the diagnosis is confirmed using the API STAPH system. Allergy testing was studied using (14) antibiotic tablets. He was an opponent of imipenem, Staphylococcus aureus bacteria. All isolates were sensitive at (100%), and the highest resistance to ciprofloxacin was at (100%), cefotaxime, imipenem, Tetracycline, ceftazidime, as they showed high resistance to the mentioned antibiotics and the reason for this was due to the bacteria's ability to produce beta-lactam enzymes. Also (50) isolates were detected out of a total of (56) isolates, and the results were that (89%) of the isolates were producing wide-spectrum beta-lactamase enzymes.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

A. E.,Brown. (2007). Benson's Microbiological Applications Laboratory Manual in General Microbiology . 10thed .McGraw-Hill comp. Inc. U.S.A.

K.P.,Talaro and B.,Chess. (2012). Foundations in Microbiology8thed . McGraw-Hill Companies.

W., Levinson. (2010). Review of medical microbiology and Immunology 11thed McGraw-Hill. New York

J.,Gottfried. ( 2005). Submitted in satis faction of both the Feed and Drug Law course requirement , and the third – year Written Work requirement . Digital Access to scholarship at Harvard: 1-7

Afrah Abd al-Ridha Ajeel, al-Maliki (2009) A study on the isolation of some types of MRSE and MRSA of patients in some Baghdad hospitals. Master's thesis, College of Science / Al-Mustansiriya University.

J.G., Collee; A.G., Fraser; B.P., Marmion and A., Simmons. (1996). Mackie and McCartney's Particle Medical Microbiology. 14th ed. Churchill Livingston. U.S.A.

V., Jarlier; M.,Nicolas ; G.,Fournier and A.,Philippon. (1988). Extended broadpectrum β-lactamase conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae : Hospital prevalence and susceptibility patterns Rev. Infect. Dis. 10(4):867-878.

Clinical, and Laboratory Standards Institute (CLSI). (2011). Performance standards for antimicrobial susceptibility testing. twenty first informational supplement M100-S21. Clinical and Laboratory Standards Institute, Wayne, U.S.A.

Hashem, Saba Talib,. (2005).Isolation and identification of bacteria contaminating burnt foods and studying the effect of fats extracted from sheep meat on their growth, Master's Thesis, College of Science, Al-Mustansiriya University.

Al-Hussein Buthaina Muhammad Taha, Abd (2009). The effect of the fluoroquinolones group on the activity of the protease enzymes and hemolysin of Staphylococcus aureus isolated from clinical.sample Master's thesis, College of Science, Al-Mustansiriya University.

A.A.A. , AL-Daoodi .( 2002) . Diagnostic and PhysiologicaL Study on Serratia marcescens isolated from infected wounds for patients bedriden in surgical wards. M.Sc. Thesis, College of science . Mosul university.

Al-Qaisi Ghada Muhammad Khalil, . (2000) A study of the enzymatic activity of Staphylococcus aureus bacteria isolated from Various inflammatory cases and their sensitivity to anti-life. Master's thesis, College of Science / Al-Mustansiriya University.

Y.H.,Haomada. (2008). Bacteriological and genetical Study on Staphylococcus aureus Isolated From wound infections . Ms.thesis, college of Education .Tikrit university.

Khazali Khitam Ali Abd 2009 Resistance of S.aureus and Pseudomonas areuginosa bacteria isolated from burn and wound infections to antibiotics and some antiseptics. Master's thesis, College of Science, Al-Mustansiriya University

A .A.,Akindele; I . K.,Adewuyi ; O.A., Adefioye ; S.A. , Adedokun and A. O ., Olaolu. ( 2010 ). Antibiogram and Beta – Lactamase Production of Staphylococcus aureus Isolates from Different Human Clinical Specimens in aTertiary Health Institution in ILe– ife , Nigeria. American –Eurasian Journal of Scientific Research.5(4) :230 – 233

K., Farzana and A.,Hameed . ( 2006) . Resistance pattern of clinical isolates of Staphylococcus aureus Against Five Groups of Antibiotics . JournaL of Research (science ), Bahauddin Zakariya University, Multan, Pakistan. 17 ( 1): 19-26.

S.A.H., AL- Babani . ( 2013) . Bacteriological and Molecular detection of Staphylococcus aureus and Its resistance to Methicillin among specimens from Kirkuk. M.Sc. Thesis, College of Science. Kirkuk university.

Ahmed Selda Said Yassin, 2008. Isolation and identification of the causes of wound infections A study of its sensitivity to antibiotics and antiseptics in Kirkuk city hospital. Master Thesis, College of Education, Tikrit University.

Abdali Yasser Adel Jabbar (2010). Extraction and purification of the catechin compound from Camella sinesis green tea and its effect on the bacteria causing urinary tract infections. Master's thesis, College of Science, Al-Mustansiriya University).

D.M., Livermore. (1995). ß – lactamases in laboratory and clinical resistance. Clincal. Microbiol. Rev. 8: 557-584.

S.P., Rao; P.S., Rama; U., Gurushanthappa; R., Manipura and K., Srinivasan. (2014). Extended spectrum beta Lactamase Producing Escherichia coli and Klebsiella Pneumoniae: AMulti –centric Study Across karnata. Journal of Laboratory Physicians. 6(1): 7-13.

M.E., Rupp and P.D., Fey. (2003). Extended spectrum β-lactamase (ESBL)- producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 63(4):353-365.