Therapeutic Comparison Between Alcoholic and Aqueous Plant Extract of Tannins with Metronidazole in Experimentally Infected Laboratory Mice Cryptosporidium parvum Oocysts

Luma Abdullatef S.N.; Firas M.B. Al-Khashab

doi:10.55544/jrasb.1.3.18

Authors

Luma Abdullatef S.N. Department of Biology, Collage of Education for Girls, University of Mosul, IRAQ.
Firas M.B. Al-Khashab Department of Biology, Collage of Education for Girls, University of Mosul, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.1.3.18

Keywords:

Cryptosporidium parvum, Cypressus sempervirens, alcohol extract, aqueous extract

Abstract

The current study was conducted during the period between from the beginning of October 2021 until the end of March 2022. The aim of the study was to measure the therapeutic efficacy of alcoholic and aqueous extracts from the local tannins Cupressus sempervirens for the treatment of experimental infected laboratory mice with Cryptosporidiosis.

Through a significant decrease in the number of Oocyst of the parasite that causes infection Cryptosporidium parvum after oral administration of the aqueous and alcoholic extracts with three concentrations (2, 1.3, 1) mg/ml compared to MTZ drug, as the alcoholic extract proved its efficacy by stopping the shedding of parasite Oocyst at the ninth day of treatment with its total absence on the eleventh day of treatment, while the shedding of parasite Oocysts in the group treated with aqueous extract stopped on the eleventh day and completely absent on the thirteenth day of infection.

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References

R. Xu et al., ‘Characterization of INS-15, a metalloprotease potentially involved in the invasion of Cryptosporidium parvum’, Microorganisms, vol. 7, no. 10, 2019, doi: 10.3390/microorganisms7100452.

F. Yu et al., ‘CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples’, Parasites and Vectors, vol. 14, no. 1, pp. 1–11, 2021, doi: 10.1186/s13071-021-04709-2.

F. S. Cunha, J. M. Peralta, and R. H. S. Peralta, ‘New insights into the detection and molecular characterization of Cryptosporidium with emphasis in Brazilian studies: A review’, Rev. Inst. Med. Trop. Sao Paulo, vol. 61, no. April, pp. 1–12, 2019, doi: 10.1590/s1678-9946201961028.

G. Certad, ‘Is Cryptosporidium a hijacker able to drive cancer cell proliferation?’, Food Waterborne Parasitol., vol. 27, no. March, p. e00153, 2022, doi: 10.1016/j.fawpar.2022.e00153.

R. A. Guy et al., ‘Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada’, Parasites and Vectors, vol. 14, no. 1, pp. 1–14, 2021, doi: 10.1186/s13071-020-04546-9.

O. Article et al., Intestinal Parasitic Infections among the Pediatric Patients in a Metropolitan City of Bangladesh with Emphasis on Cryptosporidiosis, no. March. 2022.

E. Tomczak, A. N. McDougal, and A. Clinton White, ‘Resolution of Cryptosporidiosis in Transplant Recipients: Review of the Literature and Presentation of a Renal Transplant Patient Treated with Nitazoxanide, Azithromycin, and Rifaximin’, Open Forum Infect. Dis., vol. 9, no. 1, pp. 1–5, 2022, doi: 10.1093/ofid/ofab610.

F. Murakoshi et al., ‘Nullscript inhibits Cryptosporidium and Toxoplasma growth’, Int. J. Parasitol. Drugs Drug Resist., vol. 14, no. October, pp. 159–166, 2020, doi: 10.1016/j.ijpddr.2020.10.004.

J. A. Gullicksrud et al., ‘Enterocyte–innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium’, Mucosal Immunol., vol. 15, no. 2, pp. 362–372, 2022, doi: 10.1038/s41385-021-00468-6.

G. Certad, ‘Food and Waterborne Parasitology Is Cryptosporidium a hijacker able to drive cancer cell proliferation ?’, Food Waterborne Parasitol., vol. 27, no. April, p. e00153, 2022, doi: 10.1016/j.fawpar.2022.e00153.

N. Zhang et al., ‘Prevalence and genotyping of cryptosporidium parvum in gastrointestinal cancer patients’, J. Cancer, vol. 11, no. 11, pp. 3334–3339, 2020, doi: 10.7150/jca.42393.

S. P. Sherchand, D. R. Joshi, N. Adhikari, K. P. Pant, and R. Pun, ‘Cyclosporiasis Among School Going Children of Kathmandu Valley’, Nepal J. Sci. Technol., vol. 11, pp. 193–198, 2010, doi: 10.3126/njst.v11i0.4145.

I. E. Orhan and I. Tumen, ‘Potential of Cupressus sempervirens (Mediterranean Cypress) in Health’, Mediterr. Diet An Evidence-Based Approach, no. January, pp. 639–647, 2015, doi: 10.1016/B978-0-12-407849-9.00057-9.

M. A. Farag, F. M. Harraz, H. M. Hammoda, E. Shawky, A. Mahana, and A. El-Hawiet, ‘Quantitative Nuclear Magnetic Resonance and High-Performance Thin-Layer Chromatography of Cupressuflavone as a Marker for Cupressus Species’, Rev. Bras. Farmacogn., vol. 30, no. 4, pp. 488–493, 2020, doi: 10.1007/s43450-020-00060-2.

B. G. Patgar, S. Satish, and A. R. Shabaraya, ‘Essential Oil of Cupressus Sempervirens: A Brief Review’, World J. Pharm. Pharm. Sci., vol. 10, no. 4, pp. 864–872, 2021, doi: 10.20959/wjpps20214-18713.

M. Khudhair and N. Al-Niaeemi, ‘Experimental study of heat-killed oocysts of Cryptosporidium Parvum in Balb/ c Mice’, J. Educ. Sci., vol. 29, no. 2, pp. 158–173, 2020, doi: 10.33899/edusj.2020.165305.

S. Abada, ‘Effect of garlic and pomegranate extract on rats experimentally infected with Cryptosporidium parvum and its comparison with metronidazole’, 2015.

M. Gaber, L. A. A. Galal, H. M. M. Farrag, D. M. Badary, S. S. Alkhalil, and N. Elossily, ‘The Effects of Commercially Available Syzygium aromaticum, Anethum graveolens, Lactobacillus acidophilus LB, and Zinc as Alternatives Therapy in Experimental Mice Challenged with Cryptosporidium parvum’, Infect. Drug Resist., vol. 15, no. January, pp. 171–182, 2022, doi: 10.2147/IDR.S345789.

M. Al-Khalil, ‘An epidemiological and molecular study to diagnose Cryptosporidium ssp in some areas of Dohuk Governorate’, 2017.

M. Behbahani, M. Shanehsazzadeh, and M. J. Hessami, ‘Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production’, Sci. Agric., vol. 68, no. 1, pp. 69–76, 2011, doi: 10.1590/s0103-90162011000100011.