The Optimal LD50 Gamma Ray and Sodium Azide-Induced Mutagenesis in the PBNS-86 Variety Safflower

Shafique Mirza; S. E. Mahamune; S. B. Thorat

doi:10.55544/jrasb.3.2.19

Authors

Shafique Mirza Department of Botany, Govt Vidarbha Institute of Science and Humanities, Amravati, INDIA.
S. E. Mahamune Department of Botany, Govt Vidarbha Institute of Science and Humanities, Amravati, INDIA.
S. B. Thorat Department of Botany, RDIK & NKD College, Badnera, Amravati, INDIA.

DOI:

https://doi.org/10.55544/jrasb.3.2.19

Keywords:

Safflower, Gamma ray, sodium azide, LD50

Abstract

Safflower has been grown for millennia all over the world, making it one of the most important and ancient oil-producing crops. In this work, we use gamma rays and sodium azide at LD₅₀levels to target high-yielding and desirable characters. The safflower variety PBNS-86 seeds were subjected to varying quantities of sodium azide (0.005%, 0.010%,0.015%,0.020% and 0.025%) and gamma-ray treatments at levels of 100Gy, 200Gy, 300Gy, 400Gy and 500Gy. The carcinogenic administrations of sodium azide and gamma rays had a detrimental dose-dependent association with the plant survival percentage in the PBNS-86 variety. The anticipated LD₅₀ value was calculated using probit values and fatality percentages. For gamma rays and sodium azide, the LD50 value of PBNS-86 was set at 296.2 Gy and 0.1513%, respectively. The greatest reduction in the proportion of plants that survived was induced by gamma rays and sodium azide treatments. It is determined that both mutagens are capable of causing notable changes in safflower, which may be investigated further for the purpose of mutation mapping.

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