Revelation of Specific Ferocity Genes in Escherichia coli Isolated from Patient with Urinary Tract Infections (UTIs)

Aymen Aziz Khalid; Sarwa Azeez Khaled; Huda Saleh Khuder

doi:10.55544/jrasb.3.1.40

Authors

Aymen Aziz Khalid Department of Medical Laboratory Technology, Imam Ja'afar Al-Sadiq University, Kirkuk, IRAQ. https://orcid.org/0000-0002-1332-7999
Sarwa Azeez Khaled Department of Clinical Laboratory Sciences, College of Pharmacy, University of Tikrit, Tikrit, IRAQ. https://orcid.org/0000-0002-1722-2684
Huda Saleh Khuder Department of Pharmacognosy, College of Pharmacy, University of Tikrit, Tikrit, IRAQ. https://orcid.org/0000-0003-3365-6893

DOI:

https://doi.org/10.55544/jrasb.3.1.40

Keywords:

Escherichia coli, PCR, urinary tract infection, ferocity genes

Abstract

Background: The purpose of this research is to detect the occurrence of ferocity genes in uropathogenic E. coli that isolated from urinary tract infections by Polymerase chain reaction technique.

Method: A whole of five strains of E.coli were obtained from patient with urinary tract infections. Identification of E.coli was depended on colonial morphology in culture media, gram stain, and conventional biochemical tests. ferocity factors genes (cnf-1, hly,urea A and ndv B) of E.coli strain were revelated by molecular technique.

Results: ferocity genes were detected successfully by molecular technique in five isolates of E.coli, the predominance of genes in isolates are seen in percentage 100%

Conclusion: Urinary tract infections still the major universal infection in the worldwide. The one of the most prevalent infectious diseases induce renal failure in patients is UTI that generated by UPEC. The presence of ferocity factors are critical features where the grade of pathogenicity of UPEC strains relied on it. This study indicate that was a real relationship between the ferocity factor genes of E.coli and clinical symptoms of UTI.

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