Molecular Investigation of Protein and Genes Resistance Mechanisms in Acinetobacter baumannii Isolates Recovered from Diverse Clinical Specimens in AL-Diwaniyah Province

Diyar Khlaif Flaifel

doi:10.55544/jrasb.2.3.4

Authors

Diyar Khlaif Flaifel Department of Biology, College of Sciences, University of AL-Qadisiyah, IRAQ.

DOI:

https://doi.org/10.55544/jrasb.2.3.4

Keywords:

blaOXA-51 gene, CarO- protein, AST, ESKAPE, I.C.P., Int-2, Acinetobacter baumannii

Abstract

The Search included 300 different clinical specimens from patients at Al-Diwaniyah General Teaching Hospital were collected for the study From September to December 2022. These specimens comprised blood, urine, burns, and wounds. 40(13.3%) A.baumannii out of 300 isolates. The focus of the study is to characterize the CarO protein, Int-2 and bla_oxa-51resistance mechanisms in Acinetobacter baumannii isolates recovered from different specimens. The samples were taken from various clinical specimens, which were then dispersed as follows: 40 isolates were tested for antibiotic susceptibility using the (Antibiotic Susceptibility Test-AST) on 15 (37.5%) swabs from burns, 10 (25%) swabs from wounds, 10 (25%) from urine, and 5 (12.5%) from blood samples. All isolates were found to be antibiotic-resistant, with the exception of polymyxin B, which had a 30% sensitivity rate and a 70% resistance rateThe gene Caro was found in 10 (25%) of the 40 isolates genetically analyzed using the PCR method, the gene Int-2 was found in 8 (20%) of the 40 specimens, and the gene blaOXA-51 was found in 19 (47.5%) of the 40 isolates. These results show that the gene Caro is present in proteins that encode the enzyme Carbapenemase. The findings of this investigation confirmed previous findings that bacteria have substantial polymyxin B resistance and are resistant to the majority of antibiotics. For the diagnosis of this bacteria, the blaOXA-51 gene was regarded as a diagnostic marker (A baumannii). In Conclusion Polymerase chain reaction technique was found to be simple and useful tool for detection of outer membrane protiens carO. Class 2-Integron was found to be carried by A. baumannii isolates and antibiotic resistance genes were distributed on this integron.

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